Guide to Techniques in Mouse Development : Volume 225: Guide to Techniques in Mouse Development (Methods in Enzymology, Vol 225)

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Summary: Contains the latest techniques in 1993
Review: 12 sections contains 56 chapters. As usual, Methods in Enzymology has a detailed subject INDEX and author INDEX at the end.

Section I: General methodology; anesthesia etc.
Section II: Germ cells and embryos; oocytes culture, acrosome reaction etc.
Section III: Fertilization;
Section IV: Gene expression: mRNA; quantitative analysis of mRNA
Section V: Gene expression: Reporter genes; luciferase, lacZ etc.
Section VI: Gene expression: protein; 2-D gel, immunohistochem etc.
Section VII: Gene expression: methylation; analysis of methylation by PCR etc.
Section VIII: Gene identification; cDNA library construction, cloning etc.
Section IX: Nuclear transplantation; transplantation of nuclei to oocytes etc.
Section X: Transgenic animals: pronuclear injection;
Section XI: Transgenic animals: Embryonic stem cells and gene targeting
Section XII: Lineage analysis