Enzyme Assays: A Practical Approach (The Practical Approach Series, 257)

Rating:
Summary: A good guide to the principles of enzyme assay development.
Review: Enzyme Assays: A Practical Approach is a good guide to the principles of assay development. It contains excellent chapters on the theoretical development of kinetic expressions and the statistical treatment of results. Different assays are illustrated (photometric, radiometric, chromatographic, and electochemical) in their respective chapters that include important considerations for assay development/use with each system. A chapter on buffers and protein determination is also included. Although the book provides some examples (in useful detail) of each kind of assay it is NOT a comprehensive overview. The chapters on electrochemical assays and the techniques for enzyme extraction are incomplete due only to the age of the volume (>5 years old). This book would be an excellent volume for those interested in enzyme assay development and would be suitable for a first year graduate chemistry/biochemistry course.

Rating:
Summary: A good guide to the principles of enzyme assay development.
Review: Enzyme Assays: A Practical Approach is a good guide to the principles of assay development. It contains excellent chapters on the theoretical development of kinetic expressions and the statistical treatment of results. Different assays are illustrated (photometric, radiometric, chromatographic, and electochemical) in their respective chapters that include important considerations for assay development/use with each system. A chapter on buffers and protein determination is also included. Although the book provides some examples (in useful detail) of each kind of assay it is NOT a comprehensive overview. The chapters on electrochemical assays and the techniques for enzyme extraction are incomplete due only to the age of the volume (>5 years old). This book would be an excellent volume for those interested in enzyme assay development and would be suitable for a first year graduate chemistry/biochemistry course.