Flow Cytometry and Cell Sorting (Springer Lab Manual)

Rating:
Summary: A book for the beginner and a review for the experienced
Review: This is the first book I purchased for flow cytometry after checking out 3 unsatisfactory books on the same subject from the library. I am a clinician by training and so I needed a book that explains the basic background of the method, PLUS detailed procedures to follow, and explanation on how to alter those procedures to suit one's experiment. This book is written and presented in a way that makes it easy to understand even if one has no prior trianing in flow-cytometry. It does not have so much technical jargon that a newbie would not be able to understand. Everything is clear and well-explained. I have "debugged" some of my current procedures using this book. Chapter 3 (Immunofluroescence: Basic Considerations) is a must-read: the validity of one's results seem to largely depend on factors discussed in this chapter. Included also is a list of internet resouce for cytometry, although some of websites have been outdated. Overall a very nice reference, but as in all lab procedures-related books, one book never covers everything you need to know, so I use this book by Radbruch in conjuction with others.