A Practical Guide to Membrane Protein Purification (Separation, Detection, and Characterization of Biological Macromolecules)

Rating:
Summary: A simple and much enough contents for everyone
Review: Membrane proteins have many typical characters that are very different from other water-soluble proteins. You can learn almost about topics of the membrane protein simply. This book is constructed with two big chapters and first chapter gives explanations about general strategies and techniques about purification (choice of protein sources, solubilization techniques, etc.), chromatography (hydroxylapatite, ion-exchange, chromatofocusing, metal chelate, affinity, covalent, hydrophobic, gel filtration, etc.) and electrophoresis(denaturing, native gel). In second chapter, several examples of membrane proteins were reported. Each purification procedures were detailed and you will be able to understand step by step. This book covers totally about practical membrane protein science and will be a nice guide for beginners as well as experts.

Rating:
Summary: A simple and much enough contents for everyone
Review: Membrane proteins have many typical characters that are very different from other water-soluble proteins. You can learn almost about topics of the membrane protein simply. This book is constructed with two big chapters and first chapter gives explanations about general strategies and techniques about purification (choice of protein sources, solubilization techniques, etc.), chromatography (hydroxylapatite, ion-exchange, chromatofocusing, metal chelate, affinity, covalent, hydrophobic, gel filtration, etc.) and electrophoresis(denaturing, native gel). In second chapter, several examples of membrane proteins were reported. Each purification procedures were detailed and you will be able to understand step by step. This book covers totally about practical membrane protein science and will be a nice guide for beginners as well as experts.